Composite

Part:BBa_K3102023

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-CAE-ter (secreted CAE)

This T7-RBS-CAE-TEV-hlyA-ter (secreted CAE) composite is engaged into the cellulose acetate esterase (CAE) synthetesis with the CAE part (BBa_K3102010) and secretion thanks to TEV peptide signal (BBa_K3102041) and HlyA basic parts (BBa_K554002). The CAE is able to break down cellulose acetate fibers. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 1234
    Illegal AgeI site found at 254
    Illegal AgeI site found at 341
    Illegal AgeI site found at 479
    Illegal AgeI site found at 662
    Illegal AgeI site found at 710
    Illegal AgeI site found at 878
    Illegal AgeI site found at 1091
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None